Cytotoxic and molecular outcomes of a theranostic nanosystem in breast cancer cell models

Koiti Araki – Professor, Institute of Chemistry - University of São Paulo; Robson Guimarães – Postdoc, Institute of Chemistry - University of São Paulo; Rodrigo Kawassaki – PhD Student, Institute of Chemistry - University of São Paulo; Luciana Lopes – Professor, Institute of Biomedical Sciences - University of São Paulo

Introduction: In breast cancer treatment, achieving targeted drug delivery, reducing systemic toxicity, and enabling real-time tumor monitoring remain defiant challenges [1]. Nanomedicine offers solutions through theranostic nanosystems that combine therapeutic interventions and diagnostic procedures, offering a multifaceted approach [2], [3]. In this context, a platform was developed for targeted intraductal delivery using ethosomes coencapsulating methotrexate (MTX) and iron-decorated titanium dioxide nanoparticles (UPNs) for enhanced MRI imaging [3], aiming to improve disease management beyond current limitations [2], [4].

Learning Objectives:

• Recognize the implications and advantages of using a theranostic nanosystem as a novel treatment for breast cancer.
• Acknowledge the distinct cell culture methods and their impact in obtaining reliable results and solid conclusions.