Skip to main content
CRS 2026 Annual Meeting & Exposition Main banner

Manufacturing and Process Scale-Up

Utilizing AF4 to Enable Accurate HPLC-CAD Composition Analysis of PEGylated Liposomes

Guillaume Bucher – Scientific Officer, JRC; Dora Mehn – Scientific Officer, JRC; Jessica Ponti – Scientific Officer, JRC

    Poster Presenter(s)

  • SA

    Silvia Appleton, n/a

    Researcher
    Joint Research Centre, European Commission, Lombardia, Italy

Introduction:

Given the significant role and growing popularity of lipid-based nanocarriers, especially since the COVID-19 pandemic, we propose an analytical method for the straightforward and reliable physicochemical characterisation of PEGylated lipid-based nanoparticles. This approach integrates size separation using Asymmetric Flow Field-Flow Fractionation (AF4) with composition analysis via High Performance Liquid Chromatography coupled to a Charged Aerosol Detector chromatography (HPLC-CAD). The rationale behind this strategy is to exploit AF4 for isolating liposomes from unincorporated molecules, thereby enabling reliable quality control regarding their composition.

Learning Objectives:

  • Understand the advantages of AF4 over traditional purification in overcoming analytical bias in quality control
  • Include in the analytical cascade the composition analysis of liposomes contained in AF4 fractions
  • Optimize PEG-lipid post-insertion and assess its stability by using AF4 and HPLC-CAD in combination