High Throughput Single Particle Interferometric-based Sizing and Payload Analyser for Lipid Nanoparticles

David Juncker – Professor, Department Chair; Principle Investigator, Department of Biomedical Engineering, Juncker Lab - McGill University; Andreas Wallucks – Post Doc Fellow, Juncker Lab - McGill University

Introduction:

Lipid nanoparticles (LNPs) are 50-200nm carrier vesicles, optimised^1,2 for therapeutic index.³ Unlike drugs that can be structure-optimised, LNP structure-function relationships remain largely intractable. DLS provides coarse and non-function predictive metrics⁴, in addition to being confounded by large particle biases, while NTA lacks sensitivity. Cryo-TEM can reveal function indicative structures but is low throughput and costly.  In contrast, interferometric scattering (iSCAT) imaging enables label-free single-particle sizing  (~37nm), as shown for extracellular vesicles⁵, enabling high throughput single particle LNP characterisation.