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Nanomedicine and Nanoscale Delivery (Focus Group – NND)

Storage and Stress Stability of Lipid Nanoparticles with Different Ionizable Lipids

Yvonne Perrie – Head of Institute, University of Strathclyde; Sarah Lindsay – Research Assistant, University of Strathclyde
Introduction:

The COVID-19 pandemic accelerated the development of mRNA vaccines [1], with lipid nanoparticle (LNP) encapsulated nucleoside-modified mRNA enabling the rapid approval of the Pfizer–BioNTech and Moderna vaccines [2]. Despite their high efficacy and fast development, mRNA–LNP vaccines suffer from intrinsic instability and dependence on ultra-cold storage, limiting global accessibility [3]. This study evaluates the stability of different LNP formulations stored at 4, 25, and 50 °C, focusing on temperature-induced physicochemical changes.

Learning Objectives:

  • Understand the impact of storage conditions on the physicochemical stability of LNPs and encapsulated mRNA
  • Compare methods of mRNA quantification, highlighting differences between RiboGreen assay and gel electrophoresis
  • Evaluate the relationship between LNP structural changes and functional mRNA expression in vitro and in vivo