Delivery strategy and timing shape macrophage responses to glucocorticoid-loaded PLGA nanoparticles
Susel Del Sol-Fernández – Researcher, Instituto de Ciencias Físicas, Universidad Nacional Autónoma de México, Mexico; Rafael Martín-Rapún – Professor, Instituto de Nanociencia y Materiales de Aragón, Spain; Jesús Martínez de la Fuente – Professor, Instituto de Nanociencia y Materiales de Aragón, Spain
PhD Candidate Instituto de Nanociencia y Materiales de Aragón (INMA), Spain
Introduction: Glucocorticoids are widely used to treat inflammatory diseases (1); however, their long-term use is associated with relevant adverse effects. Nanoparticle-based delivery systems have been proposed to modulate drug exposure and potentially influence cellular responses (2). In this study, hydrocortisone and dexamethasone were encapsulated in PLGA nanoparticles and evaluated using two in vitro treatment strategies to investigate how controlled release and timing of administration affect macrophage inflammatory responses.
Learning Objectives:
Understand how glucocorticoid-loaded PLGA-nanoparticles loaded influence macrophages inflammatory response.
Compare delivery-dependent effects of hydrocortisone and dexamethasone using PLGA nanoparticles
Evaluate how treatment timing modulates macrophage inflammatory gene expression under LPS stimulation
