Characterization and Safety of Biomimetic MOFs in the Raw 264.7 Cell Line

Cindy Venegas Mata – Graduate Student, South Dakota State University; Irabin Aime Fabius – Graduate Student, South Dakota State University; Jiahui Yuan – Graduate Student, South Dakota State University; Zihaho Yu – Graduate Student, Institut des Matériaux Poreux de Paris; Mathilde Lepoitevin – Assistant Professor, Institut des Matériaux Poreux de Paris; Christian Serre – Research Director, Institut des Matériaux Poreux de Paris; Samantha Sampson – Professor, Stellenbosch University; Admire Dube – Assistant Professor, University of Western Cape

Introduction:

Tuberculosis (TB) is a disease caused by a bacterial infection that prevails within the alveolar macrophages in the deep lung. TB is responsible for millions of deaths annually [1]. Metal-organic framework (MOF), MIL-88A(Fe) and MIL-100(Fe) were selected as host-directed therapy (HDT) candidates due to their tunable surface. To achieve macrophage delivery through biomimicry, biological lipids and polymers were used to coat MOFs (mycolic acid, curdlan and DPGG) at different percentages. MOFs were characterized using SEM imaging and zeta potential to evaluate surface charge. The safety of the coated and uncoated MOFs were tested using the MTT assay in RAW 264.7 Macrophages.