Freeze-drying of PLGA hybrid nanoparticles with doxycycline for pulmonary delivery

Rafaela Nanini Figueiredo – Undergraduation Student, Faculty of Pharmaceutical Sciencea, Universidade Estadual de Campinas; Alice Baldassin Mendes – Undergraduation Student, Faculty of Pharmaceutical Science, Universidade Estadual de Campinas; Laura De Oliveira Nascimento – Professor, Faculty of Pharmaceutical Science, Universidade Estadual de Campinas

Introduction: PLGA hybrid nanoparticles holds promisse for the pulmonary delivery of doxycycline (DX), allowing better treatment efficacy due to fast onset of action, deep lung targeting and high local drug concentration. However, its liquid form is unstable at room temperature, while its powder form is not straightforward to achieve. Therefore, this work is aimed at lyophilization studies to obtain a powder for resuspension. Three excipients, mannitol, trehalose and maltose and their combination with isoleucine were tested to achieve a high loaded and redispersible powder.