Lyophilization approach to improve long-term stability of LGA-PEI nanoparticles for drug delivery

Laura Chaves-Martínez – Scientific Lead, Speratum Biopharma Inc; Yendry Corrales-Ureña – Researcher, National Laboratory of Nanotechnology (LANOTEC), National Center for High Technology (CeNAT); Maria López-Brenes – Scientific Lead, Speratum Biopharma Inc; Jian-Ming Lü – Assistant Professor, Baylor College of Medicine; Christian Marín-Müller – Chief executive officer, Speratum Biopharma Inc

Introduction: Polymer-based nanoparticles offer a promising platform for nucleic acid delivery; however, their clinical application is often hindered by storage instability, limiting their therapeutic potential¹,². Lactic-co-glycolic acid-polyethylenimine (LGA-PEI)-based nanoparticles (LPNPs) demonstrate high transfection efficiency with low toxicity in vitro while ensuring robust delivery and safety in vivo³,⁴. This study evaluates a lyophilization strategy to enhance LPNP stability, addressing key storage challenges and improving their functionality, viability, and applicability in nucleic acid delivery.