Optimization of Psoriasis-like spheroids for pre-clinical screening of therapies
Marta Ferreira – CEO, Inovapotek; Vânia Rocha – PhD student, Instituto de Investigação e Inovação em Saúde; Bruno Sarmento – Group lider, Instituto de Investigação e Inovação em Saúde; Juliana Viegas – Junior researcher, Instituto de Investigação e Inovação em Saúde
PhD student Instituto de Investigação e Inovação em Saúde, Universidade do Porto Porto, Portugal
Introduction: Psoriasis vulgaris is a chronic inflammatory skin disorder affecting 2-3% of the global population. It is characterized by excessive keratinocyte proliferation and persistent inflammation which leads to erythematous plaques with silvery scales [1]. Treatment strategies for psoriasis vulgaris include topical therapies, phototherapy, systemic agents and biologic treatments [2]. The optimization of preclinical models will help to advance testing, predicting responses before moving to in vivo testing and clinical [3]. The psoriasis like spheroids will allow the observation of effects in a cell-to-cell scenario, shifts in secretome and viability.
Learning Objectives:
Understand the production of extracellular matrix components in HaCat spheroids in different culture conditions.
Understand the effect of TNF-α induction in the spheroid concerning induction of inflammation and cytokine release.
