Introduction: Polymeric carriers offer synthetically tuneable alternatives to lipid nanoparticles for small interfering RNA (siRNA) delivery, enabling control over stability, release, and biocompatibility. Unlike plasmid DNA (pDNA), siRNA requires a delicate balance between stable encapsulation and efficient cytosolic release.[1] However, structure–activity relationships (SAR) for siRNA polyplexes remain elusive due to inconsistent formulation designs and limited characterization of these properties.[2] This study addresses this gap through a robust, multi-technique characterization workflow.
Learning Objectives:
Assess polyplexes colloidal stability over time using simple DLS data.
Identify promising formulations based on controlled siRNA release.
Correlate polymeric carrier properties with functional transfection efficiency.
