PEGylation of cationic diblock polymeric nanoparticles improves physical properties and biological function
Kenneth Sims, PhD – Materials Scientist IV, Advanced Materials, Battelle Memorial Institute; Danielle Huk, PhD – Biologist IV, Life Science Research, Battelle Memorial Institute; Tony Duong, PhD – Chemical Engineer IV, Ad, Battelle Memorial Institute; Ashlee Colbert, PhD – Materials Scientist II, Advanced Materials, Battelle Memorial Institute; Emma Schmitz – Chemical Engineer II, Advanced Materials, Battelle Memorial Institute; Molly Kaufman – Biologist I, Life Science Research, Battelle Memorial Institute
Principal Scientist Battelle Memorial Inst. Columbus, Ohio, United States
Introduction: Polymeric nanoparticles (PNPs) are a promising delivery method for gene therapies. PNPs overcome known concerns for established delivery methods, such as cargo capacity limitations, adverse immune responses, manufacturing challenges, and the high cost of scaled production1. Using a novel delivery vehicle discovery and development platform, this work explored how the addition of a non-ionic material, polyethylene glycol (PEG), to PNPs could alter the binding capability of serum proteins to PNPs as a way to increase stealth and improve cell delivery performance.
Learning Objectives:
Synthesize new PEGylated PNPs with the PEG molecule covalently incorporated in each linear polymer.
Assess physical characteristics of PEGylated PNPs compared to their native forms.
Test the transfection efficiency of PEGylated PNPs in serum-rich conditions.
