Oscillation-generating micromixing (FDmiX) – a new method for the manufacturing of mRNA-lipid nanoparticles

Frank Erdmann – Post-Doc, Institute of Pharmaceutical Technologies; Julia Kollan – Research Assistant, Institute of Pharmaceutical Technologies; Henrike Lucas – Post-Doc, Institute of Pharmaceutical Technologies; Stephanie Krüger – Post-Doc, Biozentrum Halle; Karsten Mäder – Professor, Institute of Pharmaceutical Technologies

Poster Presenter(s)

Fitore Mejzini (she/her/hers)

Pharmacist
Martin-Luther University
Halle, Sachsen-Anhalt, Germany

Introduction:

For advancing LNP-based RNA therapeutics, the development of the formulation needs to be consistent across various stages of drug development. Microfluidics enables LNP production with precisely defined properties. However, large-scale manufacturing remains very limited and is primarily achieved through parallelizing microfluidic devices [1]. The use of a fluidic oscillator [2,3] (FDmiX) is a novel and very promising micromixing principle for the preparation of mRNA-LNPs. In our present work, we investigated the feasibility of upscaling the production of eGFP mRNA-LNPs by changing the size of the fluidic oscillator and by adjusting flow parameters (TFR: Total Flow Rate).

Learning Objectives:

Upon completion, the participant will discover a novel mixing technology, based on oscillation.
Upon completion, the participant will recognize the difference between low- and high-throughout LNP production.
Upon completion, the participant will distinguish between LNPs which were prepared using the FDmiX resp. a T-piece.